HomeCLC FAQ - Analyses-related questionsRNA-seqHow can I identify differential expression between groups affected by more than factor?

8.2. How can I identify differential expression between groups affected by more than factor?

The Differential Expression for RNA-Seq tool only allows for selection of one column from the metadata for Test differential expression due to:

 

For comparing groups of samples affected by several factors an additional column describing these groups should be included in the metadata.

An example of this could be a time series experiment with the factors:

  • Treatment (No treatment, Mock Treatment and Treatment)
  • Time points (0 hours, 12 hours and 24 hours)

To compare 'Treatment 12 hours' vs 'Mock treatment 12 hours' a column describing both the treatment and the time should be included in a separate column in the metadata:

For visualization purpose you may also wish to include the information is separate columns.

 

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