HomeCLC FAQ - Analyses-related questionsRNA-seqWhy do I get the error message "Inconsistent input: The provided metadata does not describe the input data." in the Differential Expression for RNA-Seq wizard?

8.4. Why do I get the error message "Inconsistent input: The provided metadata does not describe the input data." in the Differential Expression for RNA-Seq wizard?

The error message "Inconsistent input: The provided metadata does not describe the input data." indicates a mismatch between the selected metadata and the selected Expression Tracks.

 

 

 

The reasons for this can be:

  • A Metadata Table for a different experiment was selected
  • Expression Tracks (GE) or (TE) for a different experiment (Metadata Table) were selected
  • Data was never associated with the selected Metadata Table created within the Workbench
  • A copy of the Metadata Table was selected
  • The Metadata Table and associated data was moved to a different location
  • Association with the Metadata Table was lost by a persistence issue on the hard drive

The first thing to check is if the correct Metadata Table was selected (Section A). If the correct Metadata Table was selected, then continue to check if the Expression Tracks are associated with the Metadata Table (Section B).

 

Section A: Is the correct Metadata Table selected?

To check if a different Metadata Table was selected, please follow these steps:

  1. Open the window to browse to the Metadata Table in the Experimental design and comparisons step of the Differential Expression for RNA-Seq wizard.
  2. Right-click on the selected Metadata Table and click Show Location.

The selected Metadata Table will now be highlighted in the Navigation Area window of the wizard, revealing if the expected Metadata Table was indeed selected.

 

 

To solve the issue, navigate to the Metadata Table describing the selected input tracks.

By default, the Workbench always name the Metadata Table "Samples" on import. To avoid selecting the wrong Metadata Table you may rename the object with a unique name for easy recognition.

 

Section B: Check if the Expression Tracks are associated with the Metadata Table

To check if the Expression Tracks are associated with the Metadata Table please follow the steps:

  1. Open the Metadata Table.
  2. Select all rows of the table. To do this click a single row, then press Ctrl+A or ⌘+A on Mac to select all rows.
  3. Click the button labelled Find Associated Data.

 

If Expression Tracks (GE) or (TE) are associated with the Metadata Table, then continue as follows:

  1. Filter to the (GE) or (TE) tracks depending on which ones you wish to analyze.
  2. Click the button labelled Find in Navigation Area.
  3. Launch the Differential Expression for RNA-Seq tool wizard again. This time the associated Expression Tracks will be pre-selected in the wizard and the error should not show again. Hence, un-associated data must have been selected in the previous launch.

 

If getting No data found message:

 

 

The no data found message can arise for four reasons:

 

  1. The Metadata Table was created within the Workbench, but data was never associated with it.

To solve the issue, continue to associate data as described in the following manual section: Associating data elements with metadata

 

  1. A copy of the Metadata Table was selected.

If copying a Metadata Table and its associated data within a location, e.g. to move all data into a subfolder, then the copied data (Expression track etc.) will be associated with the original Metadata Table and not with the copy.

To solve the issue please deleted the copied Metadata Table and use the original. If the original Metadata Table has been thrown in the recycle bin it can be restored again. To avoid such issues in the future please move the data instead of copying it.

Relevant manual pages regarding restoring and moving data can be found at:

 

  1. If Metadata Table and associated data has been moved to a different location using older version of the Workbench.

When moving data between locations, the original data is kept. This means that you are essentially doing a copy instead of a move operation. Hence, the data moved to the new file location is associated with the Metadata Table in the original Location.

 

Thus, if moving data to a new Location, the data needs to be re-associated with the Metadata Table. Information on how to associate data with a Metadata Table is described in the following manual section: Associating data elements with metadata

From CLC Genomics Workbench version 20.0 Metadata tables can be moved to a new File Location while maintaining metadata associations.

 

  1. If you are sure data was associated with the Metadata Table and its not a copy, then try to rebuild the index for the location. If re-indexing does not solve the issue, then you will need to associate the data again.

Information on how to rebuild index is described in the following section of the manual for the Genomics Workbench and Server.

 

 

 

 

 

 

 

 

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