HomeCLC software: Important notificationsIssues affecting only versions of products released prior to June 2017RNA seq analysis on SOLiD data producing incorrect results in CLC Genomics Workbench 7.5 and 7.5.1 plus CLC Cancer Research Workbench 1.5, 1.5.1 and 1.5.2

3.11. RNA seq analysis on SOLiD data producing incorrect results in CLC Genomics Workbench 7.5 and 7.5.1 plus CLC Cancer Research Workbench 1.5, 1.5.1 and 1.5.2

Who is affected

This issue only affects users running RNA seq analysis on SOLiD data in the following Workbenches:

  • CLC Genomics Workbench 7.5 and 7.5.1
  • CLC Cancer Research Workbench 1.5, 1.5.1 and 1.5.2
  • CLC Genomics Server 6.5 and 6.5.1

 This issue was fixed with CLC Genomics Workbench 7.5.2, CLC Cancer Research Workbench 1.5.3 and corresponding CLC Servers, released Feb 17, 2015.

What are the symptoms

  • You will see reads mapping to exon-exon boundaries only. No reads map fully within one exon.
  • The number of mapped reads is substantially lower that what is expected or what was seen with previous Workbench versions.

The expression levels are incorrect and should not be trusted! Please delete the affected RNA seq result files!

 

 

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