1.4. How to import predesigned primers to the workbench?
- First make a list of the primers that you want to import in e.g. Excel.
- The first column should be the primer name and the second column should be the primer sequence. There must not be any empty rows between the primers.
- Save the file as a .csv file. This can be done by selecting CSV (comma delimited) (*.csv) under Save as type.
- Import the .csv file into your CLC Workbench using the standard import option and leaving the import setting as automatic.
The primers are now imported as a sequence list.
- To extract the primers (sequences) as individual primers go to:
Main Workbench: Toolbox | General Sequence Analysis | Extract Sequences
Genomics Workbench: Toolbox | Classical Sequence Analysis | General Sequence Analysis | Extract Sequences
Biomedical Genomics Workbench: Toolbox | Tools | Helper Tools | Extract sequences
- In the wizard that opens up chose your imported sequence list and click Next.
- In the next step of the wizard chose to Extract to single sequences and click Next.
- Choose to Save your results and finish the wizard.
Your primer sequences will now extracted as single sequences (Figure 1).
Figure 1: The sequence in the sequence list are here extracted to single sequences, i.e. individual primers.
Each primer can now be analyzed for its properties. However, please notice that only it is only possible to select one primer at the time for analyzing primer properties.
Attached is an example .csv file with 10 primer sequences.