• Make a list of the primers that you want to import in e.g. Excel. The Workbench support IUPAC codes for nucleotides as list on the following manual page: IUPAC codes for nucleotides
• The first column should be the primer name and the second column should be the primer sequence. There must not be any empty rows between the primers.
• Save the file as a .csv file. This can be done by selecting CSV (comma delimited) (*.csv) under Save as type.
• Import the .csv file into your CLC Workbench using the standard import option and leaving the import setting as automatic.

The primers are now imported as a sequence list.

• To extract the primers (sequences) as individual primers go to:

Main Workbench: Toolbox | General Sequence Analysis | Extract Sequences

Genomics Workbench: Toolbox | Classical Sequence Analysis | General Sequence Analysis | Extract Sequences

• In the wizard that opens up chose your imported sequence list and click Next.
• In the next step of the wizard chose Extract to single sequences and click Next.
• Choose to Save your results and finish the wizard.

Your primer sequences will now extracted as single sequences (Figure 1).



Figure 1: The sequence in the sequence list are here extracted to single sequences, i.e. individual primers.

Each primer can now be analyzed for its properties. However, please notice that only it is only possible to select one primer at the time for analyzing primer properties.